| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030880 |
| Chromosome: | chromosome 3 |
| Location: | 3756468 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169350 | (1 of 3) IPR007860 - DNA mismatch repair protein MutS, connector domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGGGCGCACGGCGCGCGGCGGAGAGCCACAGCTCCGCTTTGTCGGCG |
| Internal bar code: | TAACGAAACGTAAAATGGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5040 |
| LEAP-Seq percent confirming: | 81.0345 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGTGTAGCCGGCATCAG |
| Suggested primer 2: | GCGATGAGTTGATTGACGGC |