| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030899 |
| Chromosome: | chromosome 1 |
| Location: | 584741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003500 | CLUB1,MSH2 | (1 of 1) IPR017987 - Wilm's tumour protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGGGGCGGGAGCCGGAATGTCTGGGGGTCTGGGGATCCTGGGGGCT |
| Internal bar code: | TGCTAAAGGAACCGTGGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2217 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCCGCTACTCTAACCT |
| Suggested primer 2: | TCGACCACACTGCTCAGAAC |