| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.030903 |
| Chromosome: | chromosome 13 |
| Location: | 2516942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580050 | (1 of 2) PTHR23074//PTHR23074:SF20 - AAA ATPASE // GH08677P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCGTTCTCACCCGGCCCGAGTACTCGTCCGCCCGGTGGCTGGACGGC |
| Internal bar code: | CCTATTAAGCTACTTGCGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1197 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATTTGGGGTGTACGCG |
| Suggested primer 2: | CCAATCAAGGCACAAACGCA |