Insertion junction: CLIP2.030907_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.030907
Chromosome:chromosome 6
Location:8226910
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g306450 (1 of 2) 3.1.1.75 - Poly(3-hydroxybutyrate) depolymerase / Poly(HA) depolymerase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACCCGTGGCCACGTGCTGCCACCACTGCTACTGCCACTGTCAGAAGT
Internal bar code:CTTTGTGGAGAACACCCTACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCGTCTTGTCTTCCTGC
Suggested primer 2:TTCGCATCACCACCACATCA