| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.030918 |
| Chromosome: | chromosome 16 |
| Location: | 2275659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658725 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCACCAGCAGCGCCGCCATGCACCGCCAGATTACGGCGGGGCCGGAC |
| Internal bar code: | TCACGAAAGTTTCCCTCGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3996 |
| LEAP-Seq percent confirming: | 92.6829 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGTCAACGAGCCGCAAC |
| Suggested primer 2: | CAGCAGTAAGACTGGGGGAG |