Insertion junction: CLIP2.030933_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.030933
Chromosome:chromosome 7
Location:6067576
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g355300 BES5 (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAACCCCGTGCAACACACATCCCCTCATGACGCCTGCACTCCCGCCAC
Internal bar code:AGTGTGAGTCGATTGTCCAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:6801
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGGTCCAAGCAAAG
Suggested primer 2:GATCGCTTTGGTGATGTCGC