| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.030945 |
| Chromosome: | chromosome 14 |
| Location: | 3619620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACGTACATCATGTTTACATCGTGAAACGGCTTCCCTCTGCTTGCCAT |
| Internal bar code: | GAGTGGTCGGTCTACATTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2576 |
| LEAP-Seq percent confirming: | 79.5181 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGCGGTGTTGGGTAAG |
| Suggested primer 2: | TCGGCAAAGTCATTTCGGGA |