| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030947 |
| Chromosome: | chromosome 4 |
| Location: | 3734869 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229350 | (1 of 1) 2.1.1.290 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase / tRNA-yW synthesizing enzyme-4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAAACATGGTCCACGCCGGCATGCATTCCTAGTCAATAGCGTACCTC |
| Internal bar code: | GATCCATTACTCTATTGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2355 |
| LEAP-Seq percent confirming: | 92.7711 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCGCAGAGAAAATGCA |
| Suggested primer 2: | ATTTGGAAAGCGGCTCAGGA |