| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030950 |
| Chromosome: | chromosome 12 |
| Location: | 9802887 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548152 | (1 of 1) K15084 - solute carrier family 25 (mitochondrial carrier protein), member 16 (SLC25A16, GDA, LEU5) | 3'UTR | |
| Cre12.g548577 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAAGAACCGCCCCCACCTCCAGCAGACGCGAACGAGATCCTTGATCTG |
| Internal bar code: | AAATTTAAGTTTGATCATTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1340 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGGCCTGTGTAGACTT |
| Suggested primer 2: | CCAGAGAGAACAGCGACCAG |