Insertion junction: CLIP2.030951_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030951
Chromosome:chromosome 6
Location:6254762
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292249 PHC50 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGTCCCCGGTGACCACTGCCTCCGGCGGTGAGTTGTCTACCTCCACCT
Internal bar code:CGACCTTCTTCCCGCGCGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:759
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGTCGCCAGTCAGTGAA
Suggested primer 2:CTTTGGACACGCTGTGGTTG