| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030953 |
| Chromosome: | chromosome 6 |
| Location: | 7325283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299500 | intron | ||
| Cre06.g299550 | ZIP6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCGACACTCTCCGCCACGTGTGTGCCGCCAGACCATGTCGGACCTG |
| Internal bar code: | TTTATTATGTCTAGGATACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1208 |
| LEAP-Seq percent confirming: | 72.3684 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGAACCTCGAAACCACA |
| Suggested primer 2: | TAAGAAGAAGCAGGCGGTGG |