| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030969 |
| Chromosome: | chromosome 15 |
| Location: | 5346487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGTGCCGCGGTCAATTGTTGCGCAGGCTGCGGAGCTGCACCAGCTGC |
| Internal bar code: | CCAAGCATACATCTTGGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3616 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGCCATGTATGAACTG |
| Suggested primer 2: | GACATCGCTTTCCATGTCGC |