| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030981 |
| Chromosome: | chromosome 8 |
| Location: | 2940722 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374700 | FAP244 | (1 of 2) PTHR14885:SF1 - WD REPEAT-CONTAINING PROTEIN 96; Flagellar Associated Protein 244 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGGCGTTGCAGCAGCGGTTGGCGGCGGAGGGCGAGGCGCGCTACGAG |
| Internal bar code: | GTAAAGGAATGCAGATGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1756 |
| LEAP-Seq percent confirming: | 38.961 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATTGGGGTGATGAAGGC |
| Suggested primer 2: | TGCTCACCCTCTCTGGCTAT |