Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.030989 |
Chromosome: | chromosome 8 |
Location: | 4033307 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g382650 | UBQ4 | (1 of 2) K09313 - homeobox protein cut-like (CUTL); Bi-ubiquitin | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCCGCCAAAGTTGGGGGGTCGGTCCTCGTCCCTGGGGGTCCGTCGG |
Internal bar code: | TCACCGCCTCGGCTACACAATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2030 |
LEAP-Seq percent confirming: | 57.1429 |
LEAP-Seq n confirming: | 4 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTGACGAATGGACAATCG |
Suggested primer 2: | GGTTGCTCCAGTCGCTTACT |