| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031003 |
| Chromosome: | chromosome 17 |
| Location: | 3772296 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726700 | (1 of 4) 6.2.1.1 - Acetate--CoA ligase / Acyl-activating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACGCACACGCACACGCCGGCCCCCCCACGCACCATCAGCCAGT |
| Internal bar code: | CCGGTCCTCGCTGGCAGGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4671 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCTGTCGCACTGGACAC |
| Suggested primer 2: | CTGTCCTGTAGCCCTCTCCT |