Insertion junction: CLIP2.031023_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031023
Chromosome:chromosome 12
Location:7457619
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g556911 MRE11 Double-strand break repair protein MRE11; (1 of 1) K10865 - double-strand break repair protein MRE11 (MRE11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCACCCTGTCCACCCTCCCGCCCCCGGCGCCGCCGGCTCACCTTCTC
Internal bar code:TCGGAATACCGTTCAGGCCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGGCTGGTCAACTACTTT
Suggested primer 2:AACATCCTCCTCCCTCCTCC