| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.031045 |
| Chromosome: | chromosome 11 |
| Location: | 4056910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480750 | (1 of 1) 3.4.19.5//3.5.1.1 - Beta-aspartyl-peptidase // Asparaginase / L-asparagine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGAGATCAGTGGCCCTGACTCCCCTGCGCGCCCACTGCGGCACCCCAC |
| Internal bar code: | ACAACGTCATGGTAGATAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGTGTGACAGGCATAC |
| Suggested primer 2: | CTACTGCCGTTGTATTGCGC |