Insertion junction: CLIP2.031048_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031048
Chromosome:chromosome 16
Location:4767178
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g687850 PTK22 (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype; Protein of unknown function intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTGGCCCTAGGCGTGTCTTGCAGTACAGAGGCTGCAAGCAGGCGGCA
Internal bar code:GTCGCGGGGCGTAGTCAAGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:5205
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGACATACGGAGTCGGTG
Suggested primer 2:CTCACTTTGTCACTTGGCGC