| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031050 |
| Chromosome: | chromosome 12 |
| Location: | 3608166 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513100 | (1 of 1) 3.2.1.165 - Exo-1,4-beta-D-glucosaminidase / GlcNase | CDS | |
| Cre12.g801394 | (1 of 8) IPR001163//IPR010920 - LSM domain, eukaryotic/archaea-type // LSM domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCATGTCTGTGGACTACCGGGAACCCTACACGGCCACACTGAATGGCG |
| Internal bar code: | GTTATGCGCGTGGGCCTCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAACAGCTGGTCCTCGT |
| Suggested primer 2: | CCCTCCACCAGCTTCAACAT |