| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.031061 |
| Chromosome: | chromosome 3 |
| Location: | 2286876 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157725 | PQQ1 | Putative quinoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAACCAGTTTATCAAGGGCTGCACCAGGTACTCCGCCGCTGTCTCGT |
| Internal bar code: | CGGCTTCGGGGGGGTACCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4046 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAACACAATTGGCACGCC |
| Suggested primer 2: | TCGAAGGTGTCAAGAGGCAC |