| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031061 |
| Chromosome: | chromosome 3 |
| Location: | 2286878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157725 | PQQ1 | Putative quinoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGCACGTGTGAGTTCGCGACCCGGGAGCAGCAACACTGACTCGTGA |
| Internal bar code: | TAAGGAAAAAGTTATGGTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3733 |
| LEAP-Seq percent confirming: | 91.4286 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAAGGTGTCAAGAGGCAC |
| Suggested primer 2: | AAGAACACAATTGGCACGCC |