| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031063 |
| Chromosome: | chromosome 17 |
| Location: | 1100911 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703900 | IFT172,SLB | (1 of 1) PTHR15722:SF2 - INTRAFLAGELLAR TRANSPORT PROTEIN 172 HOMOLOG; Intraflagellar Transport Protein 172 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGTGCCAACAAAGGGGGGAGGGGCTAGCGCACGGCTATGCACCTCGG |
| Internal bar code: | CAGGGGCCGCCTTACATTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4842 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCACGGCTGACTTGCTCA |
| Suggested primer 2: | CAACTTCCCAGCAGAGGAGG |