Insertion junction: CLIP2.031075_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031075
Chromosome:chromosome 15
Location:804297
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g637761 ABCD1 Peroxisomal long-chain acyl-CoA transporter, ABC superfamily; (1 of 1) K05677 - ATP-binding cassette, subfamily D (ALD), member 3 (ABCD3, PMP70) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCTGTAACCCCAACCTCCGCCTCGCCCCAACTCCCAACACCTCCCAAC
Internal bar code:AACTAATCATGGGGTCCTCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCACACCGAACACAAACA
Suggested primer 2:GTGTGCAAGTTGCCTGACAG