| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.031082 |
| Chromosome: | chromosome 12 |
| Location: | 8662566 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548051 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCCTCTGGAAGCGGAGGCCACCAGGGGCGGCAGCAGCCACTGCCCA |
| Internal bar code: | CAGGCTGCATTTCTGGGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3809 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGACTCAAGGATGGTC |
| Suggested primer 2: | ACTCGCTCTGCCGAAGAAAA |