| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031082 |
| Chromosome: | chromosome 12 |
| Location: | 8662567 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548051 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCATAGAGGCCTGGGCCCGTGCCAGCGTCGGCGCACCCAGCTGGCG |
| Internal bar code: | CCAGGGGGAAGCCGTTTTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3182 |
| LEAP-Seq percent confirming: | 95.5882 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGCTCTGCCGAAGAAAA |
| Suggested primer 2: | GCTGGGACTCAAGGATGGTC |