Insertion junction: CLIP2.031118_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031118
Chromosome:chromosome 15
Location:1299694
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639504 (1 of 3) PF13637//PF13920 - Ankyrin repeats (many copies) (Ank_4) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGTGACTACTGAAGGTGACTACCGTAGGTGACTACCGTAGGTGACTA
Internal bar code:GAATACTGGAGGAGGGTCCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2189
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCGGGTTAGAGGGAATCG
Suggested primer 2:TAGGTTGGAAGGAGGAGGGG