| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.031125 |
| Chromosome: | chromosome 4 |
| Location: | 2519628 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACATCCGCCACCACCACAGGGCCGGCTAGCAGAATGCCCTTGCCCTGC |
| Internal bar code: | GTGTTTTCTCGCTGCAAAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 31.4286 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATTGATTGGCTGCTGAGC |
| Suggested primer 2: | CATCAAAGGCAGCGACATCG |