| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031129 |
| Chromosome: | chromosome 12 |
| Location: | 1414855 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488752 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTTTAAGACCTGACGAGCTGTAGCAGGGGCGAGGCCGCCGGATGAG |
| Internal bar code: | CGGTTCGCGAACGGGCTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2817 |
| LEAP-Seq percent confirming: | 67.4419 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCATTCTGGCGTTCCA |
| Suggested primer 2: | CCAGCAGTGTCTGTTGTTGC |