Insertion junction: CLIP2.031140_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031140
Chromosome:chromosome 14
Location:2318003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g622850 RAD51A,RAD51 (1 of 1) K04482 - DNA repair protein RAD51 (RAD51); RAS associated with diabetes protein 51 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAGTCGAAGTGACAGAAACGCATTTGGAAATCCAACATCAAAAGACC
Internal bar code:ATAATACAGTGAGGGCGGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3901
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCGCTTGTATTATTGGC
Suggested primer 2:ATGTGATGCTGGGCGAGAAA