| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031141 |
| Chromosome: | chromosome 3 |
| Location: | 8446257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212865 | (1 of 3) IPR002638 - Quinolinate phosphoribosyl transferase, C-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAAACACCGATGGTGGCTGCGCCCCCTTCGGGCGCGTCCCTTGCCGG |
| Internal bar code: | GTCATGGAGCCCCACTATAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 156 |
| LEAP-Seq percent confirming: | 34.2857 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGTAGCGCAGTGTTTGT |
| Suggested primer 2: | CTACACGTGACACCCCTCAC |