Insertion junction: CLIP2.031159_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031159
Chromosome:chromosome 2
Location:265231
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g074800 CYG9 (1 of 1) IPR000700//IPR001054//IPR029787 - PAS-associated, C-terminal // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACCGACGGCGCGGAGGACTCTCTCACCACCGACACCGCCCTGCACAG
Internal bar code:AATCATCGGAATTAGACGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2662
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGATCGGAGATCGCTCAA
Suggested primer 2:GGCAATCGCATAAGCACCTG