Insertion junction: CLIP2.031163_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.031163
Chromosome:chromosome 3
Location:3075645
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g164050 KIN15-1,KIN15A,KIN12-1 Kinesin motor protein; (1 of 2) K10400 - kinesin family member 15 (KIF15) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCAGCCGCGACTCGCGCTCCGCCAGCTGCTCGCGGCTGGAGGCCAGG
Internal bar code:ATTATTCCCGAAAAACAATGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2593
LEAP-Seq percent confirming:97.5
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCTTGCTAGGGTGTCACC
Suggested primer 2:AAACCTGCCAACAATCGCAC