| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031180 |
| Chromosome: | chromosome 12 |
| Location: | 467475 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494650 | CSV10 | Chlamydomonas-specific family protein; (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCCCTCCGCCGCACTGCAGCCACCCGGCGAGCCCATACGCATGTCC |
| Internal bar code: | AAACTGAAATTTTCGCGCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1752 |
| LEAP-Seq percent confirming: | 67.6923 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAACGAGGCAGAGGACA |
| Suggested primer 2: | TTGTTTGCCTGCGTGTTAGC |