| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.031237 |
| Chromosome: | chromosome 12 |
| Location: | 8541712 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549150 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTCGGACGCGGGGTTTGCCTGTTGCGGGGCCCGTTTGCGAGCCGTGT |
| Internal bar code: | CGCGTAGATCCCTGGCTCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1873 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGAAGGTTCGACACAGA |
| Suggested primer 2: | CCGACTCCTGCTGCTAACAA |