Insertion junction: CLIP2.031243_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031243
Chromosome:chromosome 2
Location:4946138
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g110200 THO1 Oligoendopeptidase; (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACTCAGAAGCCGTTTGGTGCGCCCACACCAGACGTACGTTGCTGAAGC
Internal bar code:GGAGTCGTATTACTGGACACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2880
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCGCATTCGTTGTATGCC
Suggested primer 2:CACGAACTGTCTCGGTCACA