Insertion junction: CLIP2.031260_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031260
Chromosome:chromosome 5
Location:3163202
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g232002 ACX2 Acyl-CoA oxidase/dehydrogenase; (1 of 2) PTHR10909//PTHR10909:SF317 - ELECTRON TRANSPORT OXIDOREDUCTASE // ACYL-COENZYME A OXIDASE 2, PEROXISOMAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCCACCCCTGGCGCGGACCGTCCTAGAAGATCTTTACTGTGGTCCAG
Internal bar code:AGTGTGCTCTATCTCTCTCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:483
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TACAAGGTGGTGAGCACGAC
Suggested primer 2:ACACACACACACGGAGCTAG