Insertion junction: CLIP2.031276_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.031276
Chromosome:chromosome 2
Location:474056
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g076200 (1 of 1) 3.4.11.2 - Membrane alanyl aminopeptidase / Peptidase E CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGCGTCCGTCACGGGCACCGCCTGCAACCGGTCGGACCCCGGCTCG
Internal bar code:CATCTAGGAAAATACCGCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTGCCGTTGCTGCTACT
Suggested primer 2:GGCAGTTCCGTGTTTCACAC