| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.031284 |
| Chromosome: | chromosome 11 |
| Location: | 357845 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467576 | (1 of 1) PTHR33403//PTHR33403:SF5 - FAMILY NOT NAMED // PROTEIN SPIRAL1-LIKE 2-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTCACGCTAGCTGCCCAGCCGCATCCAAGCAAGCCTTAGCAGGGACG |
| Internal bar code: | GTTAGCGAGTGGTGTAATTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1540 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGCTTGTGAGGCCTCT |
| Suggested primer 2: | TAAACACCCGCAGTTACGCT |