Insertion junction: CLIP2.031298_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031298
Chromosome:chromosome 1
Location:691359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g003850 CYP1,CYP747A1 Cytochrome P450, CYP197 superfamily; (1 of 19) 1.14.14.1 - Unspecific monooxygenase / Xenobiotic monooxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTATCTTGGGTGGCGAGACGGGGTGTTGCGGATGTGTCGGAGGATCGC
Internal bar code:GGGATGACACACCTTGGTAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1696
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTCAAGATTCGCCTGCG
Suggested primer 2:GGCACCAGGGAACATACACA