| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031310 |
| Chromosome: | chromosome 1 |
| Location: | 366605 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800003 | (1 of 24) IPR006311 - Twin-arginine translocation pathway, signal sequence | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGAGGAGGGCGCGGAGGCCGGGTAGGTCGAAGCGGGGCCGGGGGCCG |
| Internal bar code: | GTCGCGCGTTAAGATGGTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1439 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCAAATACAGCAGCC |
| Suggested primer 2: | CGCTGCTGCATTTGATGGTT |