Insertion junction: CLIP2.031311_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031311
Chromosome:chromosome 6
Location:3295059
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g277350 HDA13 RPD3/HDA1 type histone deacetylase; (1 of 4) K06067 - histone deacetylase 1/2 (HDAC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTAGAGGTATGCCTGTGCGTCGGTCGCGGAGCTCGCTCCCGCCTCGCG
Internal bar code:GTGGCTAGATAAATTTCCTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGCTACACAACTGTTCCT
Suggested primer 2:TCAACCACACGGAACACACA