Insertion junction: CLIP2.031316_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031316
Chromosome:chromosome 2
Location:6808761
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388134 (1 of 1) PTHR13398//PTHR13398:SF0 - GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2 // GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTTGTGGCTGCTTGCTGACTCGCTGCCGCCGCTCGCTACCGCATGCTC
Internal bar code:CAATCTAACGTTCCCGCATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:974
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGAACGTGTCGGGGTTG
Suggested primer 2:CAAGGTTACACAAACGCCCG