| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031318 |
| Chromosome: | chromosome 16 |
| Location: | 2106762 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657700 | (1 of 2) PF13661 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_4) | CDS | |
| Cre16.g657800 | CCD3,CCD2 | (1 of 2) K00464 - all-trans-8'-apo-beta-carotenal 15,15'-oxygenase (diox1); Carotenoid cleavage dioxygenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCAATGCAATTTTGTCAGTTTGATTGCATGTTGCAACACTCGGTATT |
| Internal bar code: | TGGGCAGTTAGAGGCAACTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3090 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCTAGCAATTGGGGCC |
| Suggested primer 2: | AAAACCCATCAAACGCGGTG |