| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031322 |
| Chromosome: | chromosome 9 |
| Location: | 376049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749947 | FAP21 | Flagellar Associated Protein 21; (1 of 1) PTHR24343//PTHR24343:SF158 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGATGACATTAAGAAGTACCGGCAAAGCACGGTGCACGAGCCGGGTA |
| Internal bar code: | TAAGTAGGTAGTAATGATAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCATATCCATAGCGCC |
| Suggested primer 2: | CATGGGCGTCCTAGGGTAAC |