| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031326 |
| Chromosome: | chromosome 1 |
| Location: | 583089 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003500 | CLUB1,MSH2 | (1 of 1) IPR017987 - Wilm's tumour protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGTGGAGGAGCAGGTGCTGGTTGTGTGTGTGTGTGTGTGTGACATG |
| Internal bar code: | AGACGGCCTCGGTCCCGCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1304 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCCTTGACAATCCCCC |
| Suggested primer 2: | TACTCGGTCAACATGGCCAC |