| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.031346 |
| Chromosome: | chromosome 12 |
| Location: | 6526370 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537900 | (1 of 1) PF11927 - Protein of unknown function (DUF3445) (DUF3445) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTTCCTTGTGCTCTACGCGCAGCTTCTACGACGCACCGCCGGGGCCG |
| Internal bar code: | CATTCCGGTAGTTCGCTATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1422 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGGAAACCGTAGTCACA |
| Suggested primer 2: | ACGTGGCGGAATCAGAAAGT |