Insertion junction: CLIP2.031357_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031357
Chromosome:chromosome 14
Location:1335519
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTTGTTTCTGGCACACAATCCGGGTGATGGCGGCCGCAGACAATGCC
Internal bar code:TACACCAACACTATTTTAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1292
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GAAACCACCTGCGAGGATGA
Suggested primer 2:CTACCATCCTCTCTGCGCTG