Insertion junction: CLIP2.031367_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031367
Chromosome:chromosome 6
Location:6160326
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291800 COQ2 (1 of 1) 2.5.1.39 - 4-hydroxybenzoate polyprenyltransferase / 4-hydroxybenzoate nonaprenyltransferase; Para-hydroxybenzoate-polyprenyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTGTGTTTGCGGTTTGGTTTGGTGTGCGTGTGTGTGTGGTGCGTGAAT
Internal bar code:GACGGGCAATGGCAGGTTATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:888
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCTCAACGACTACAGGT
Suggested primer 2:CAAAGCAGCTGCACACCATT