| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.031381 |
| Chromosome: | chromosome 16 |
| Location: | 2391952 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659600 | (1 of 1) K14834 - nucleolar complex protein 3 (NOC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCAAGTGTTTGCCGAAGGAGCGACCTAGGGGGAGGGAGCAGGGGCTT |
| Internal bar code: | TATGACCCTAGTGCTTGAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 46.2687 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACTAGGGGGCTTGAGGG |
| Suggested primer 2: | GCAGCTTACTATGCGCACAC |