Insertion junction: CLIP2.031406_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031406
Chromosome:chromosome 12
Location:2850065
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g503200 (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGATGTTAAAGCCAGGCGCCTCAGCAGTGGGGTTGTAAGGGCGGACCGG
Internal bar code:CGTGCGGCCCTCGCATTAACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3402
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGAGGCCGTCGTCAACAT
Suggested primer 2:CTCGTTGGTCCGAATCCACA